Costus pictus D. Don leaf extract stimulates GLP-1 secretion from GLUTag L-cells and has cytoprotective effects in BRIN-BD11 ß-cells.

ETHNOPHARMACOLOGICAL RELEVANCE: Costus pictus D. Don, commonly known as insulin plant, is a traditional Indian antidiabetic herbal medicine with glucose-lowering and insulin secretory effects having been reported in animal models and humans with Type 2 diabetes. However, its effects on GLP-1 secretion from intestinal endocrine L-cells and potential metabolic and protective effects in insulin secreting pancreatic ß-cells are not yet fully understood. AIM OF THE STUDY: This study is aimed to elucidate the effects of Costus pictus D. Don leaf extract (CPE) on L-cell function and GLP-1 secretion using the established murine GLUTag L-cell model and to investigate its potential cytoprotective effects against detrimental effects of palmitate and cytokines in pancreatic ß-cells using BRIN-BD11 cells. METHODS: Costus pictus D. Don dried leaf powder was extracted by soxhlet method. Cell viability was determined by MTT assay. Changes in gene and protein expression were quantified by qPCR and western blotting, respectively. GLP-1 and insulin secretion were measured by ELISA. RESULTS: CPE significantly enhanced the percentage of viable BRIN-BD11 and GLUTag cells and protected BRIN-BD11 cells against palmitate- and proinflammatory cytokine-induced toxicity. CPE enhanced acute GLP-1 secretion 6.4-16.3-fold from GLUTag cells at both low (1.1 mM) and high (16.7 mM) glucose (P < 0.01) concentrations. Antioxidant (Nrf2, Cat & Gpx1) and pro-proliferative (Erk1 and Jnk1) gene expression were upregulated by 24 h culture with CPE, while proinflammatory transcription factor NF-κB was downregulated. CONCLUSION: Diminished postprandial GLP-1 secretion and loss of insulin secreting ß-cells are known contributors of T2DM. Our data suggests that CPE acutely stimulates GLP-1 secretion from L-cells. Long term exposure of the BRIN-BD11 cells to CPE enhances cell number and may protect against palmitate and proinflammatory cytokines by activating multiple pathways. Thus, the current study suggests that the possible antidiabetic properties of CPE may be linked to enhanced GLP-1 secretion and ß-cell protection which could be beneficial in the management of T2DM.

Adsorption of 2-chloropyridine on oxides--an infrared spectroscopic study.

The adsorption of 2-chloropyridine on SiO(2), TiO(2), ZrO(2), SiO(2)-Al(2)O(3) and H-mordenite has been studied by IR spectroscopy. The different modes of interaction with oxide surfaces, i.e. hydrogen-bonding and adsorption at Brønsted or Lewis acid sites, was modelled by ab initio calculations at the B3LYP/DZ+(d) level. Adsorption on SiO(2) results in hydrogen bonding to surface hydroxyl groups, whereas the spectra obtained following adsorption on TiO(2) and ZrO(2) display evidence for electron transfer at Lewis acidic surface sites. Protonation of 2-chloropyridine at Brønsted acidic sites was detected only for adsorption on SiO(2)-Al(2)O(3) and H-mordenite, indicating the presence of Brønsted acidic sites on these oxide surfaces with pK(a) values

IR spectroscopy of N-methylpyrrole adsorbed on oxides--a probe of surface acidity.

IR spectra of N-methylpyrrole (NMP) have been measured following adsorption on, and subsequent desorption from, SiO(2), TiO(2), ZrO(2), SiO(2)-Al(2)O(3), H-mordenite, and sepiolite. Three modes of adsorption have been observed: (i) hydrogen bonding to surface hydroxyl groups, (ii) electron transfer at Lewis acidic surface sites, and (iii) proton transfer at Brønsted acidic surface sites. Protonation of NMP was detected only for adsorption on SiO(2)-Al(2)O(3) and H-mordenite, indicating the presence of Brønsted acidic sites with pK(a) values